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2021/01/13

Femtopath Citation Update

Enhanced anticancer activity and endocytic mechanisms by polymeric nanocarriers of n‑butylidenephthalide in leukemia cells

Author
X.‑F. Huang, P.‑T. Chen, Y.‑L. Lin, M.‑S. Lee, K.‑F. Chang, K.‑W. Liao, G.‑T. Sheu, M.‑C. Hsieh and N.‑M. Tsai.

Purpose
The purpose of this study was to investigate the antitumor mechanisms of n-butylidenephthalide (BP) and to further examine the delivery efficacy of polycationic liposome containing PEI and polyethylene glycol complex (LPPC)-encapsulated BP in leukemia cells.

Methods
MTS, flow cytometric and TUNEL assays were performed to assess cell viability and apoptosis. BP and BP/LPPC complex delivery efficiency was analyzed by full-wavelength fluorescent scanner and fluorescence microscope. The expressions of cell cycle- and apoptosis-related proteins were conducted by Western blotting.

Results
The results showed that BP inhibited leukemia cell growth by inducing cell cycle arrest and cell apoptosis. LPPCencapsulated BP rapidly induced endocytic pathway activation, resulting in the internalization of BP into leukemia cells, causing cell apoptosis within 1 h.

Conclusions
LPPC encapsulation enhanced the cytotoxic activity of BP and did not influence the effects of BP induction that suggested LPPC-encapsulated BP might be developed as anti-leukemia drugs in future.

Keywords
Leukemia, Polycationic liposome containing PEI and polyethylene glycol complex (LPPC), n-Butylidenephthalide (BP), Cell apoptosis, Cell cycle

Reference
in Transl Oncol (2020). https://doi.org/10.1007/s12094-020-02500-w

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