Product

Product

FemtoPath EGFR Mutation Screen Kit

Epidermal growth factor receptor (EGFR) is one of the most frequently amplified, mutated and/or overexpressed genes in human malignancies. Inactivation of EGFR kinase with tyrosine kinase inhibitors (TKIs), such as gefitinib (Iressa) and erlotinib (Tarceva), suppresses signal transduction pathways which control tumor cell growth, proliferation, and resistance to apoptosis. TKIs therapy have shown a significant symptom benefit and prolong survival on the treatment of Non-Small Cell Lung Cancer (NSCLC) patients with somatics mutations in the EGFR gene. Therefore, detection of the somatic mutations in the EGFR gene may provide a more accurate response to the cancer patients receiving TKIs therapy.

FemtoPath EGFR Mutation Screen Kit is a PCR-based test to enrich somatic mutations of EGFR gene in DNA.
With direct sanger sequencing, FemtoPath EGFR Mutation Screen Kit is able to detect 1% somatic mutation from DNA derived from formalin-fixed paraffin-embedded (FFPET), fine needle biopsy or pleural effusion specimens.

The feature of FemtoPath mutation screen kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required

01

Product Specification

  • Gene Name

    EGFR epidermal growth factor receptor

  • Catalog

    20 tests: EGFR-101-0020
    50 tests: EGFR-101-0050

  • Official Symbol

    EGFR

  • Alternative Name

    EGFR; Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; ERBB1; HER1; mENA; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine protein kinase ErbB1; Receptor tyrosineprotein kinase ErbB-1; Urogastrone; wa2; Wa5; proto-oncogene c-ErbB -1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; ERBB; HER1; PIG61

  • GeneID

    1956

  • Uniprot ID

    P00533

  • Target Codon

    Exon 18: G719
    Exon 19: Exon 19 insertion/ deletion
    Exon 20: S768 / Insertion mutation/ T790
    Exon 21: L858 / L861

  • Other Interested Codons

    Exon 18: any insertion and deletion between 711~728 aa
    Exon 19: any insertion and deletion between 739~760 aa
    Exon 20: V769/ D770/ P772/ H773/ V774/ R776/ C797/ any insertion and deletion between 761~823 aa
    Exon 21: any insertion and deletion between 850~875 aa

  • Platform

    PCR + Sanger sequencing assay

  • Covered Gene Region

    Exon 18: 711~728 aa; 
    Exon 19: 739~760 aa; 
    Exon 20: 761~823 aa; 
    Exon 20 T790: 782~823 aa; 
    Exon 21: 850~875 aa

  • List of components

    1. EGFR Exon 18 PCR Primer Mix
    2. EGFR Exon 19 PCR Primer Mix
    3. EGFR Exon 20 PCR Primer Mix
    4. EGFR Exon 21 PCR Primer Mix
    5. EGFR Exon 20 T790 PCR Primer Mix
    6. EGFR Exon 18 Sequencing Primer-r
    7. EGFR Exon19 Sequencing Primer-r
    8. EGFR Exon 20 Sequencing Primer-r
    9. EGFR Exon 21 Sequencing Primer-r
    10. EGFR Exon 20 T790 Sequencing Primer-r
    11. DNA Quality Control Primer Mix
    12. 2x PCR Master Mix
    13. EGFR Exon 19 wild type plasmid
    14. EGFR Exon 20 wild-type plasmid 
    15. EGFR Exon 21 wild-type plasmid
    16. EGFR Exon 19 deletion plasmid 
    17. Nuclease-Free Water

  • Recommend PCR Condition

    Stage 1: 95°C, 10mins (x1 cycles)
    Stage 2: 94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
    Stage 3: 72°C, 10mins° (x1 cycles)
                    10°C, ∞

  • Shipping Condition

    4°C

  • Long Term Storage

    Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles.

  • Datasheet

    FemtoPath EGFR Mutation Screen Kit datasheet

  • Order Now

02

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