Product

Product

FemtoPath BRAF Mutation Screen Kit for Real-Time PCR

BRAF, a kinase-encoding gene from the RAS/RAF/ MEK/ERK pathway, recently has being studied in all malignant melanomas, including thyroid cancer, malignant melanoma, ovarian cancer, colorectal cancer.  The most common mutations in BRAF occur at the valine at residue 600 (V600) within exon 15, and it were suggested to be a predictive biomarker which shows limited response to anti-EGFR therapy. 

FemtoPath BRAF Mutation Screen Kit is a PCR-based test to enrich somatic mutations of BRAF gene in DNA. 
With direct sanger sequencing, FemtoPath BRAF Mutation Screen Kit is able to detect 1% somatic mutation from DNA derived from formalin-fixed paraffin-embedded (FFPET), fine needle biopsy or pleural effusion specimens.

The feature of FemtoPath mutation screen kit:
1. Open system and easy to be done
2. No post-PCR manipulation required
3. Accuracy
2. Minimal sample size

01

Product Specification

  • Gene Name

    BRAF v-raf murine sarcoma viral oncogene homolog B1

  • Catalog

    24 tests: BRAF-201-0024

  • Official Symbol

    BRAF

  • Alternative Name

    BRAF; v-raf murine sarcoma viral oncogene homolog B1; serine/threonine-protein kinase B-raf; BRAF1; p94; 94 kDa B-raf protein; proto-oncogene B-Raf; murine sarcoma viral (v-raf) oncogene homolog B1; B-Raf proto-oncogene serine/threonine-protein kinase (p94); NS7; RAFB1; B-RAF1

  • GeneID

    673

  • Uniprot ID

    P15056

  • Target Codon

    Mutation

    Base Change

     

    V600E

    c.1799T>A

     

    V600Ecomplex

    c.1799_1780GT>AA

     

    V600K

    c.1798_1799GT>AA

     
  • Platform

    Real-Time PCR system
    Compatible with ABI7500、 ABIViiA 7、 Qiagen Rotor-Gene Q、 Roche LightCycler 480

  • List of components

    1. Reagent A: PCR buffer
    2. Reagent B-E/Ec: V600E/Ec probe
    3. Reagent B-K: V600K probe
    4. Reagent B-S768I: S768I probe
    5. Enzyme A: Tag MasterMix
    6. Enzmye B: dUTP 
    7. Positive control 
    8. Negative control

  • Shipping Condition

    4°C

  • Long Term Storage

    Stable for up to one year when store at -20°C. 
    After opening, stable for up to six month. 
    Avoid freeze/thaw cycles. (Less than 5 times)

  • Intended Use

    For Research Use Only

  • Datasheet

    FemtoPath BRAF Mutation Screen Kit datasheet (Real-Time PCR system)

  • Order Now

02

Other Product Of BRAF

ProductCategory

  1. ABL

    BCR/ABL1 Dual Color Dual Fusion FISH ProbeABL Mutation Screen KitABL Exon 4 Primer Set (A)ABL Exon 4 Primer Set (B)ABL Exon 6 Primer Set (A)ABL Exon 6 Primer Set (B)

  2. AKT3

    AKT3 Mutation Screen KitAKT3 Exon 2 Primer Set

  3. BRAF

    Real-Time BRAF V600 Mutation KitBRAF Mutation Screen KitBRAF Exon 15 Primer Set

  4. EGFR

    EGFR C797S Mutation Screen KitReal-Time EGFR Mutation KitEGFR Mutation Screen KitEGFR Exon 18 Primer SetEGFR Exon 19 Primer SetEGFR Exon 20 Primer SetEGFR Exon 20 T790 Primer SetEGFR Exon 21 Primer SetEGFR/CEN 7 Dual Color FISH Probe

  5. FLT3

    FLT3 Mutation Screen KitFLT3 Exon 20 Primer Set

  6. HRAS

    HRAS Mutation Screen KitHRAS Exon 2 Primer SetHRAS Exon 3 Primer SetHRAS Exon 4 Primer Set (A)HRAS Exon 4 Primer Set (B)

  7. JAK2

    JAK2 Mutation Screen KitJAK2 Exon 14 Primer Set

  8. KIT

    KIT Mutation Screen KitKIT Exon 11 Primer Set (A)KIT Exon 11 Primer Set (B)KIT Exon 13 Primer Set

  9. KRAS

    KRAS Mutation Screen KitKRAS Exon 2 Primer SetKRAS Exon 3 Primer SetKRAS Exon 4 Primer Set (A)KRAS Exon 4 Primer Set (B)

  10. NRAS

    NRAS Mutation Screen KitNRAS Exon 2 Primer SetNRAS Exon 3 Primer SetNRAS Exon 4 Primer Set (A)NRAS Exon 4 Primer Set (B)

  11. PIK3CA

    PIK3CA/CEN 3 Dual Color FISH ProbePIK3CA Mutation Screen KitPIK3CA Exon 9 Primer SetPIK3CA Exon 20 Primer Set

  12. TP53

    TP53/CEN 17 Dual Color FISH ProbeTP53 Mutation Screen KitTP53 Exon 8 Primer Set

  13. ASXL1

    ASXL1 Mutation Screen KitASXL1 Primer Set

  14. DNMT3A

    DNMT3A Mutation Screen KitDNMT3A Exon 22 Primer Set

  15. SF3B1

    SF3B1 Mutation Screen KitSF3B1 Primer Set

  16. TET2

    TET2 Mutation Screen KitTET2 Exon 1 Primer Set

  17. MED12

    MED12 Mutation Screen KitMED12 Exon 2 Primer Set

  18. PTEN

    PTEN Mutation Screen KitPTEN Exon 7 Primer Set

  19. PIK3CD

    PIK3CD Mutation Screen KitPIK3CD Exon 22 Primer Set

  20. CALR

    CALR Mutation Screen KitCALR Exon 9 Primer Set

  21. PDGFRA

    PDGFRA Exon 18 Primer SetPDGFRA Exon 14 Primer SetPDGFRA Exon 12 Primer Set

  22. IDH1

    IDH1 Mutation Screen KitIDH1 Exon 4 Primer Set

  23. IDH2

    IDH2 Exon 4 Primer Set (B)IDH2 Exon 4 Primer Set (A)

  24. MPL

    MPL Exon 10 Primer Set

  25. MDM2

    MDM2/CEN 12 Dual Color FISH Probe

  26. CDKN2A

    CDKN2A/CEN 9 Dual Color FISH Probe

  27. CCND1

    CCND1/IGH Dual Color Dual Fusion FISH ProbeCCND1 Dual Color Break Apart FISH ProbeCCND1/CEN 11 Dual Color FISH Probe

  28. FGFR1

    FGFR1/CEN 8 Dual Color FISH Probe

  29. 1p36

    1p36/1q25 Dual Color FISH Probe

  30. VHL

    VHL/CEN 3 Dual Color FISH Probe

  31. MET

    MET/CEN 7 Dual Color FISH Probe

  32. MYC

    MYC/CEN 8 Dual Color FISH Probe

  33. DDIT3

    DDIT3 Dual Color Break Apart FISH Probe

  34. EGR1

    EGR1/5p15 Dual Color FISH Probe

  35. IGH

    CCND1/IGH Dual Color Dual Fusion FISH ProbeBCL2/IGH Dual Color Dual Fusion FISH ProbeIGH Dual Color Break Apart FISH Probe

  36. RUNX1

    ETV6/RUNX1 Dual Color Dual Fusion FISH ProbeRUNX1/RUNX1T1 Dual Color Dual Fusion FISH Probe

  37. PML

    PML/RARA Dual Color Dual Fusion FISH Probe

  38. BCL2

    BCL2/IGH Dual Color Dual Fusion FISH Probe

  39. COL1A1

    COL1A1 Dual Color Break Apart FISH ProbeCOL1A1/PDGFB Dual Color Dual Fusion FISH Probe

  40. PDGFB

    PDGFB Dual Color Break Apart FISH ProbeCOL1A1/PDGFB Dual Color Dual Fusion FISH Probe

  41. FGFR2

    FGFR2/CEN 10 Dual Color FISH Probe

  42. ALK

    ALK Dual Color Break Apart FISH Probe

  43. FUS

    FUS Dual Color Break Apart FISH Probe

  44. FOXO1

    FOXO1 Dual Color Break Apart FISH Probe

  45. MYB

    MYB Dual Color Break Apart FISH Probe

  46. ROS1

    ROS1 Dual Color Break Apart FISH Probe

  47. RET

    RET Dual Color Break Apart FISH Probe

  48. BIRC3

    BIRC3/MALT1 Dual Color Dual Fusion FISH Probe

  49. ETV6

    ETV6/RUNX1 Dual Color Dual Fusion FISH Probe

loading