FemtoPath EGFR Mutation Screen Kit

Epidermal growth factor receptor (EGFR) is one of the most frequently amplified, mutated and/or overexpressed genes in human malignancies. Inactivation of EGFR kinase with tyrosine kinase inhibitors (TKIs), such as gefitinib (Iressa) and erlotinib (Tarceva), suppresses signal transduction pathways which control tumor cell growth, proliferation, and resistance to apoptosis. TKIs therapy have shown a significant symptom benefit and prolong survival on the treatment of Non-Small Cell Lung Cancer (NSCLC) patients with somatics mutations in the EGFR gene. Therefore, detection of the somatic mutations in the EGFR gene may provide a more accurate response to the cancer patients receiving TKIs therapy.

FemtoPath EGFR Mutation Screen Kit is a PCR-based test to enrich somatic mutations of EGFR gene in DNA.
With direct sanger sequencing, FemtoPath EGFR Mutation Screen Kit is able to detect 1% somatic mutation from DNA derived from formalin-fixed paraffin-embedded (FFPET), fine needle biopsy or pleural effusion specimens.

The feature of FemtoPath mutation screen kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required


Product Specification

  • Gene Name

    EGFR epidermal growth factor receptor

  • Catalog

    20 tests: EGFR-101-0020
    50 tests: EGFR-101-0050

  • Official Symbol


  • Alternative Name

    EGFR; Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; ERBB1; HER1; mENA; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine protein kinase ErbB1; Receptor tyrosineprotein kinase ErbB-1; Urogastrone; wa2; Wa5; proto-oncogene c-ErbB -1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; ERBB; HER1; PIG61

  • GeneID


  • Uniprot ID


  • Target Codon

    Exon 18: G719
    Exon 19: Exon 19 insertion/ deletion
    Exon 20: S768 / Insertion mutation/ T790
    Exon 21: L858 / L861

  • Other Interested Codons

    Exon 18: any insertion and deletion between 711~728 aa
    Exon 19: any insertion and deletion between 739~760 aa
    Exon 20: V769/ D770/ P772/ H773/ V774/ R776/ C797/ any insertion and deletion between 761~823 aa
    Exon 21: any insertion and deletion between 850~875 aa

  • Platform

    PCR + Sanger sequencing assay

  • Covered Gene Region

    Exon 18: 711~728 aa; 
    Exon 19: 739~760 aa; 
    Exon 20: 761~823 aa; 
    Exon 20 T790: 782~823 aa; 
    Exon 21: 850~875 aa

  • List of components

    1. EGFR Exon 18 PCR Primer Mix
    2. EGFR Exon 19 PCR Primer Mix
    3. EGFR Exon 20 PCR Primer Mix
    4. EGFR Exon 21 PCR Primer Mix
    5. EGFR Exon 20 T790 PCR Primer Mix
    6. EGFR Exon 18 Sequencing Primer-r
    7. EGFR Exon19 Sequencing Primer-r
    8. EGFR Exon 20 Sequencing Primer-r
    9. EGFR Exon 21 Sequencing Primer-r
    10. EGFR Exon 20 T790 Sequencing Primer-r
    11. DNA Quality Control Primer Mix
    12. 2x PCR Master Mix
    13. EGFR Exon 19 wild type plasmid
    14. EGFR Exon 20 wild-type plasmid 
    15. EGFR Exon 21 wild-type plasmid
    16. EGFR Exon 19 deletion plasmid 
    17. Nuclease-Free Water

  • Recommend PCR Condition

    Stage 1: 95°C, 10mins (x1 cycles)
    Stage 2: 94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
    Stage 3: 72°C, 10mins° (x1 cycles)
                    10°C, ∞

  • Shipping Condition


  • Long Term Storage

    Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles.

  • Datasheet

    FemtoPath EGFR Mutation Screen Kit datasheet

  • Order Now


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  1. Plasmid Controls

    Plasmid Controls

  2. Data Analysis


  3. NGS Cancer Panels

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  4. Service


  5. FISH Probes

    Chromosome 1Chromosome 2Chromosome 3Chromosome 5Chromosome 6Chromosome 7Chromosome 8Chromosome 9Chromosome 10Chromosome 11Chromosome 12Chromosome 13Chromosome 14Chromosome 15Chromosome 16Chromosome 17Chromosome 18Chromosome 19Chromosome 21Chromosome 22Chromosome X

  6. Real-time PCR

    Real-Time CYP2C19 Genotyping HRM KitReal-Time BRAF V600 Mutation Kit

  7. DNA methylation assay

  8. Antibodies

    MART-1 (Melan A) AntibodyCytomegalovirus (CMV) Antibodyc-Myc AntibodyClusterin/Apolipoprotein J AntibodyCTLA-4 AntibodySurvivin AntibodyStathmin AntibodyProgesterone Receptor Antibodyp57 kip2 Antibodyp21 Antibody AntibodyOct-4 AntibodyNF Kappa B/p50 AntibodyNapsin A AntibodyMSH6 AntibodyEBV LMP-1 AntibodyFlt-1/VEGFR1 AntibodyCD99 AntibodyCA 19-9 AntibodyBRAF V600E AntibodyAEG1 AntibodyCD56 AntibodyCD15/Leu-M1 AntibodyCEA AntibodyDOG1 AntibodyGLUT1 AntibodyGlycophorin A AntibodyHairy Cell Leukemia AntibodyhENT1 AntibodyMDR3 AntibodyMyogenin AntibodyN-cadherin AntibodyKBA.62 (Melanoma Associated Antigen) Antibodyp16 Antibodyp40 Antibodyp63 Antibodys-100 AntibodySOX2 AntibodySOX-11 AntibodyEstrogen Receptor AntibodyTTF-1 AntibodyBG8 LewisY AntibodyActin, Smooth Muscle AntibodyCD45R AntibodyVimentin AntibodyTIM3 AntibodyThyroglobulin AntibodyThymidylate Synthase AntibodyTFE3 AntibodyTau AntibodyTAG-72 AntibodySOX10 AntibodySALL4 AntibodyPSAP AntibodyPSA AntibodyPodoplanin AntibodyPMS2 AntibodyPD-L1 AntibodyPD-1 AntibodyPAX8 AntibodyPAX-5 Antibodyp504s Antibodyp53 AntibodyNerve Growth Factor Receptor (NGFR) AntibodyNanog AntibodyMUC6 AntibodyMUC5AC AntibodyMSH2 AntibodyMLH1 AntibodyLMO2 AntibodyKi-67 AntibodyKappa AntibodyIDH1 R132H AntibodyHER2/neu AntibodyGATA3 AntibodyFSH AntibodyFibronectinE-cadherin AntibodyCytokeratin 19 AntibodyCytokeratin 18 AntibodyCOX2Collagen Type IV AntibodyCDX-2 AntibodyCD138 AntibodyCD57 AntibodyCD45(LCA) AntibodyCD44 AntibodyCD5 AntibodyCD2 AntibodyBSEP AntibodyArginase-1 AntibodyAnnexin A1 AntibodyCA-125 Antibody